virology 4 you
in scientific fiction and actuality virus is a June premiere virus was probably the most mysterious topic in medication bacterial filter discovery electron microscope we unveil this thriller
on this course we talk about historical past of discovery virus construction virus classifications virus multiplication virus pathogenesis zoonosis some essential viruses hepatitis
Rabies is a vaccine preventable, zoonotic, viral illness. As soon as scientific signs seem, rabies is. Vaccinating canine is probably the most
value efficient technique for stopping rabies in individuals. publish
publicity prophylaxis Put up publicity prophylaxis(PEP) is the speedy remedy of a chew sufferer after rabies publicity. This prevents virus entry into
the central nervous system, which leads to imminent loss of life. PEP consists of:•In depth washing and native remedy of the chew wound or
scratch as quickly as potential after a suspected publicity;•a course of potent and efficient rabies vaccine and the administration of rabies immunoglobulin (RIG), if indicated
Laboratory prognosis of viral infections v1-Sampling
3-Nucleic acid based mostly strategies
polymerase chain response
sequencingv4-Microscopy based mostly strategies immunofluorescence Electron microscopy 5-Host antibody detection 6-Hemagglutination assay
sampling temperatures (often 4 °C) to protect the virus and stop bacterial or fungal development. Typically a number of websites can also be sampled.
Forms of samples embody the next All kinds of samples can be utilized for virologic testing. The kind of pattern despatched to the laboratory usually relies on the kind of viral an infection being identified and the take a look at required Correct sampling approach is crucial to keep away from potential pre-analytical errors .For instance and saved at applicable saved at applicable temperatures (often 4 °C) to protect the virus and stop bacterial or fungal development. 1.Nasopharyngeal swab
2.Blood pores and skin. Sputum, gargles and bronchial washings .Urine .Semen Faces .Cerebrospinal fluid .Tissues biopsies or autopsy
Viruses are sometimes remoted from the preliminary affected person pattern. This enables the virus pattern to be grown into bigger portions and permits a bigger variety of exams to be run on them. That is significantly essential for samples that comprise new or uncommon viruses for which diagnostic exams usually are not but developed Many viruses may be grown in cell tradition within the lab.
To do that, the virus pattern is blended with cells, a course of referred to as adsorption, after which the cells change into contaminated and produce extra copies of the virus Though totally different viruses usually solely develop in sure forms of cells, there are cells that help the expansion of a giant number of viruses and are a very good start line, for instance, the African monkey kidney cell line (Vero cells), human lung fibroblasts (MRC-5), and human epidermoid carcinoma cells (HEp-2). One technique of figuring out whether or not the cells are efficiently replicating the virus is to verify for a change in cell morphology or for the presence of cell loss of life utilizing a microscope Different viruses could require different strategies for development such because the inoculation of embryonated hen eggs
(e.g. avian influenza viruses
Molecular techniques are the most specific and sensitive diagnostic tests
They are capable of detecting either the whole viral genome or parts of the viral genome.
In the past nucleic acid tests have mainly been used as a secondary test to confirm positive serological results However, as they become cheaper and more automated, they are increasingly becoming the primary tool for diagnostics Polymerase chain reaction[
Detection of viral RNA and DNA genomes can be performed using polymerase chain reaction. This technique makes many copies of the virus genome using virus-specific probes. Variations of PCR such as nested reverse transcriptase PCR and real time PCR can also be used to determine viral loads in patient serum. This is often used to monitor treatment success in HIV cases. Sequencing[
Main article: Whole genome sequencing
Sequencing is the only diagnostic method that will provide the full sequence of a virus genome. Hence, it provides the most information about very small differences between two viruses that would look the same using other diagnostic tests
Viral replication is the formation of biological viruses during the infection process in the target host cells. Viruses must first get into the cell before viral replication can occur.
Through the generation of abundant copies of its genome and packaging these copies, the virus continues infecting new hosts. Replication between viruses is greatly varied and depends on the type of genes involved in them Most DNA viruses assemble in the nucleus while most RNA viruses develop solely in cytoplasm. viruses multiply only in living cells. The host cell must provide the energy and synthetic machinery and the low- molecular-weight precursors for the synthesis of viral proteins and nucleic acids
virus life cycle
4.Transcription / mRNA production,
5.Synthesis of virus components,
6.Virion assembly and
Viral replication of a bacteriophage
7- Launch (Liberation Stage).
Attachment It is step one of viral replication. The virus attaches to the cell membrane of the host cell. It then injects its DNA or RNA into the host to provoke an infection..
In animal cells these viruses get into the cell by means of the method of endocytosis which works by means of fusing of the virus and fusing of the viral envelope with the cell membrane of the animal cell
The cell membrane of the host cell invaginates the virus particle, enclosing it in a pinocytotic vacuole. This protects the cell from antibodies like in the case of the HIV virus.
(from lysosomes) strip off the virus protein coat. This releases or renders accessible the virus nucleic acid or genome Transcription / mRNA production
For some RNA viruses, the infecting RNA produces messenger RNA (mRNA), which may translate the genome into protein merchandise
For viruses with destructive stranded RNA, or DNA, viruses are produced by transcription then translation.
The mRNA is used to instruct the host cell to make virus elements. The virus takes benefit of the prevailing cell buildings to duplicate itself
synthesis of virus elements[
The elements are manufactured by the virus utilizing the host’s current organelles:
•Viral proteins: Viral mRNA is translated on mobile ribosomes into two forms of viral protein:
•Structural: proteins which make up the virus particle
•Nonstructural: proteins not discovered within the virus particle, primarily enzymes for virus genome replication
Viral nucleic acid (genome replication): New viral genomes are synthesized; templates are both the parental genome or newly fashioned complementary strands, within the case of single-stranded genomes. These genomes are made by both a viral polymerase or (in some DNA viruses) a mobile enzyme, significantly in quickly dividing cells
Virion meeting A virion is solely an lively or intact virus particle. On this stage, newly synthesized genome (nucleic acid), and proteins are assembled to type new virus particles.
This may increasingly happen within the cell’s nucleus, cytoplasm, or at plasma membrane for many developed viruses.
Launch (liberation stage
Launch (liberation stage) The viruses, now being mature are launched by both sudden rupture of the cell, or gradual extrusion (pressure out) of enveloped viruses by means of the cell membrane.
The brand new viruses could invade or assault different cells, or stay dormant within the cell.
Within the case of bacterial viruses, the discharge of progeny virions takes place by lysis of the contaminated bacterium. Nevertheless, within the case of animal viruses, launch often happens with out cell lysis. Viruses are classed into 7 forms of genes, every of which has its personal households of viruses, which in flip have differing replication methods themselves David Baltimore, a Nobel Prize-winning biologist, devised a system referred to as the Baltimore Classification System to categorise totally different viruses based mostly on their distinctive replication technique. There are seven totally different replication methods based mostly on this technique (Baltimore Class I, II, III, IV, V, VI, VII). The seven courses of Class 1: Double-stranded DNA viruses This kind of virus often should enter the host nucleus earlier than it is ready to replicate. A few of these viruses require host cell polymerases to duplicate their genome, whereas others, similar to adenoviruses or herpes viruses, encode their very own replication components.
class 2: Single-stranded DNA viruses
Viruses that fall underneath this class embody ones that aren’t as well-studied, however nonetheless do pertain extremely to vertebrates. Two examples embody the Circoviridae and Parvoviridae. They replicate throughout the nucleus, and type a double-stranded DNA intermediate throughout replication
Class 3: Double-stranded RNA viruses
. This class consists of two main households, the Reoviridae and Birnaviridae. Replication is monocistronic and consists of particular person, segmented genomes, that means that every of the genes codes for just one protein, in contrast to different viruses, which exhibit extra advanced translation Class
4: Single-stranded RNA viruses – positive-sense
The positive-sense RNA viruses and certainly all genes outlined as positive-sense may be immediately accessed by host ribosomes to right away type proteins.
may be divided into two teams, each of which replicate within the cytoplasm:
•Viruses with polycistronic mRNA the place the genome RNA kinds the mRNA and is translated right into a polyprotein product that’s subsequently cleaved to type the mature proteins. Because of this the gene can make the most of just a few strategies through which to provide proteins from the identical strand of RNA, decreasing the dimensions of its genome.
•Viruses with advanced transcription,
• for which sub genomic mRNAs,
• ribosomal frameshifting and proteolytic processing of polyproteins could also be used. All of that are totally different mechanisms with which to provide proteins from the identical strand of RNA.
Class 6: Optimistic-sense single-stranded RNA viruses that replicate by means of a DNA intermediate
A well-studied household of this class of viruses embody the retroviruses. One defining function is the usage of reverse transcriptase to transform the positive-sense RNA into DNA. As an alternative of utilizing the RNA for templates of proteins, they use DNA to create the templates, which is spliced into the host genome utilizing integrase. Replication can then start with the assistance of the host cell’s polymerases
class 7: Double-stranded DNA viruses that replicate by means of a single-stranded RNA intermediate This small group of viruses, exemplified by the Hepatitis B virus, have a double-stranded, gapped genome that’s subsequently stuffed in to type a covalently closed circle (ccc DNA) that serves as a template for manufacturing of viral mRNAs and a sub genomic RNA. The progenome RNA serves as template for the viral reverse transcriptase and for manufacturing of the DNA genome.